OXPHOS coupling and uncoupling
DOI:
https://doi.org/10.26124/bec.2024-0005Keywords:
electron transfer capacity, LEAK respiration, mitochondria, oxidative phosphorylationAbstract
Coupling of OXPHOS is classically studied by comparing ADP-stimulated O2 flux (OXPHOS capacity P at saturating ADP and Pi concentrations) with non-phosphorylating LEAK respiration L, maintained mainly to compensate for the proton leak at high chemiosmotic potential in mitochondrial preparations supplied with fuel substrates to support mitochondrial electron transfer. However, measurement of the non-coupled electron transfer capacity E (ET capacity at collapsed chemiosmotic potential) yields additional information on coupling, since ET capacity may exceed OXPHOS capacity.
Cite:
Cardoso LHD, Gnaiger E (2024) OXPHOS coupling and uncoupling. Bioenerg Commun 2024.5. https://doi.org/10.26124/bec.2024-0005
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Copyright (c) 2024 Luiza Helena Daltro Cardoso, Erich Gnaiger
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